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. 2017 Aug 16;7:8432. doi: 10.1038/s41598-017-08157-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic analyses of NOS proteins in chordates. Bayesian inference-based phylogenetic tree of chordate NOS proteins. Numbers on nodes represent posterior probabilities values. The red box includes cephalochordate proteins, including 3 closely related species of Branchiostoma (B. lanceolatum, B. floridae and B. belcheri), plus Asymmetron lucayanum. The blue box contains two ascidian species, Ciona intestinalis and Ciona savignyi. Vertebrate NOS, represented here by Homo sapiens, are highlighted by the yellow box. The NOS protein of the sea urchin Strongylocentrotus purpuratus (Ambulacraria) was used as an outgroup.