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. 2017 Aug 16;7:8489. doi: 10.1038/s41598-017-08277-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolic pathways that generate various lactate isotopomers and isotopologues in cells, traced with [1,2-¹³C₂]glucose. 4T1 cells were incubated in serum-free RPMI 1640 medium containing 6 mM [1,2-¹³C₂]glucose for 12 hours in a humidified CO₂ incubator and culture supernatant was collected for LC-MS analysis as described in Materials and Methods. The figure explain the generation of various lactate isotopologues from [1,2-¹³C₂]glucose.