FIG 1.

(A) BRIG analysis of the mcr-3-carrying plasmid pWJ1. Comparative analysis of pWJ1 with four closely related mcr-1-harboring plasmids from E. coli isolates using the BLAST Ring Image Generator. The concentric rings display similarity between the reference sequence in the inner ring and the other sequences in the outer rings. The various color levels indicate a BLAST result with a matched degree of shared regions, as shown to the right of the ring. (B) Comparison of the genetic environments of mcr-3 genes in different plasmids and shotgun sequences extracted from the GenBank database. Arrows indicate the positions and directions of the genes; Δ indicates the truncated gene. Regions with >99% homology are indicated in gray shadow, with homology of >85% shown by a lighter gray shadow.