Figure 6.
Human meniscal fibrochondrocytes (MFC) deposit functional matrix on aligned nanofibrous scaffolds. Culture-expanded meniscal fibrochondrocytes (A) derived from 10 donors took on an elongated morphology when seeded onto aligned scaffolds (B) (original magnification 20×). During 10 weeks of culture, seeded constructs developed a nonlinear stress-strain response greater than unseeded controls (USC) (C). This increase in properties corresponded well with newly deposited collagen content (D). Although donor-to-donor variability was noted, all donors significantly increased in mechanical properties over the culture period (E).
