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. 2017 Jun 27;29(7):1709–1725. doi: 10.1105/tpc.16.00796

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© 2017 American Society of Plant Biologists. All rights reserved.

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Figure 5. — Plastidial Compartmentation of Polyprenols.

(A) Analysis of total polyisoprenoids. Intact chloroplasts (Cp) were isolated from Arabidopsis leaves and fractioned into envelope (Ev), stroma (St), and thylakoid (Tk) compartments by sucrose gradient centrifugation and analyzed for the presence of polyprenols (P-9 to P-11). Representative HPLC/UV chromatograms are presented and the vertical lines indicate the retention time of the main polyprenols (P-9, P-10, and P-11) that are present in the various compartments. Extracts were supplemented with an internal polyprenol (P-14) standard prior to extraction.

(B) Quantification of polyprenols (P-9 to P-11). Polyprenol content of intact chloroplasts (Cp) and envelope (Ev), stroma (St), and thylakoid (Tk) compartments were measured in the wild type (Col-0), three independent RNAi lines (RNAi), and an overexpression line (CPT7-OE). Note that the polyprenols present in intact chloroplasts mainly accumulate in the thylakoid and envelope compartments. Values (±sd) represent the mean of three independent experiments. Statistically significant differences compared with wild-type plants are indicated; *P < 0.05 by Student’s t test.