Table 2. The Content of Plastidial Isoprenoids in Rosette Leaves and Purified Thylakoids from the Plant Lines Used in This Study.
Rosette Leaf (µg g FW−1) |
Thylakoids (µg mg Chl−1) |
|||||||
---|---|---|---|---|---|---|---|---|
Wild Type | cpt7−/− | RNAi | CPT7-OE | Wild Type | cpt7−/− | RNAi | CPT7-OE | |
Tocopherols | 8.21 ± 1.76 | 7.68 ± 0.66 | 9.56 ± 2.87 | 9.71 ± 4.28 | 3.23 ± 0.32 | 3.95 ± 0.88 | 3.87 ± 1.23 | 3.25 ± 0.86 |
Carotenoids | 203.36 ± 35.26 | 217.07 ± 21.21 | 211.05 ± 23.10 | 215.08 ± 27.71 | 232.29 ± 14.58 | 177.07 ± 20.24 | 221.64 ± 66.77 | 184.38 ± 26.56 |
Plastoquinone | 35.22 ± 3.61 | 33.82 ± 1.48 | 39.9 ± 9.71 | 32.6 ± 2.03 | 60.97 ± 8.51 | 52.22 ± 20.72 | 74.28 ± 27.77 | 63.87 ± 16.46 |
Phylloquinone | 1.63 ± 0.29 | 1.49 ± 0.27 | 1.62 ± 0.15 | 1.47 ± 0.48 | 2.38 ± 0.55 | 2.86 ± 0.51 | 2.37 ± 1.36 | 2.77 ± 0.25 |
The quantities of tocopherols, carotenoids, plastoquinone, and phylloquinone in the rosette leaves (per gram fresh weight [FW]) and purified thylakoids (per milligram of chlorophyll [Chl]) were determined from the indicated plant lines. Data are the means of at least three independent determinations ± sd, and bold numbers indicate significant difference (Student’s t test, P < 0.05) when comparing the quantity of various isoprenoids to those found in wild-type samples.