Figure 3.

Epistatic Relationship between ATRX and CAF-1 or HIR Histone Chaperone Complexes.

(A) Representative 7-week-old F3 sister plants grown on soil.

(B) Quantification of seed content in hira-1, hira-1 atrx-1/ATRX, atrx-1, and atrx-1 hira-1/HIRA mutant siliques. Mean seed number was calculated from at least 30 siliques pooled from four plants grown at the same time. A Student’s t test revealed no statistically significant difference between the genotypes.

(C) and (D) Representative 3-week-old F3 plants derived from crosses of atrx-1 alleles with ubn2-2 (C) or cabin1-2 (D) grown on soil.

(E) Quantification of total rosette surface area of the F3 sister plants described in (C) and (D). Mean rosette area was calculated from at least six 2-week-old plants for each genotype. Student’s t test in comparison to ubn2-2 ATRX for UBN2 atrx-1 and ubn2-2 atrx-1 mutants and in comparison to cabin1-2 ATRX for CABIN1 atrx-1 and cabin1-2 atrx-1 mutants; *P < 0.05.

(F) Quantification of seed content in siliques of FAS2 ATRX, fas2-5 ATRX, fas2-5 atrx-1/ATRX, and fas2-5 atrx-1 F2 sister plants. Mean for seed number was calculated from at least 30 siliques pooled from four plants grown at the same time. Student’s t test in comparison to fas2-5 ATRX; ***P < 0.001.

(G) Representative dissected siliques from fas2-5 ATRX and fas2-5 atrx-1 F2 sister plants. Red arrows indicate unfertilized ovules.

Error bars of all panels represent se of the mean.