Figure 11.

ITC Analysis of Interactions between PTST2 and Different Soluble Glucans.

(A) ITC profile of maltoheptaose (blue) and β-cyclodextrin (black) injected into a solution of the full-length His-PTST2 protein. The upper panels show the raw calorimetric data. The bottom plots are integrated heats as a function of the glucan/PTST2 molar ratio. The best fit for β-cyclodextrin was obtained from a nonlinear least squares method using a one-site binding model for two significantly distant N-values. Heats of dilution without PTST2 protein have been obtained from independent titration experiments (Supplemental Figure 7A). Reactions are exothermic.

(B) As for (A), but showing maltoheptaose (blue) and β-cyclodextrin (black) injected into the isolated CBM48 domain.

(C) As for (A), but showing maltodecaose injected into the full-length His-PTST2 protein.