Table 7.
Analysis of energy metabolism in line Kctd1 I27N
| Test | Parameter | Heterozygous mutant males | Wild-type males | Heterozygous mutant females | Wild-type females | Geno-type: P-value |
|---|---|---|---|---|---|---|
| Indirect calori-metry | Body weight (g) | 28.3 ± 1.2 | 29.2 ± 1.7 | 24.1 ± 1.6 | 24.0 ± 2.7 | |
| Food intake (g) | 0.9 ± 0.5 | 1.2 ± 0.6 | 1.4 ± 0.8 | 1.7 ± 0.7 | ||
| Average VO2 consumption (ml/h) | 86 ± 6 | 88 ± 8 | 82 ± 10 | 82 ± 13 | ||
| Average RER (VCO2/VO2) | 0.79 ± 0.03 | 0.81 ± 0.02 | 0.81 ± 0.04 | 0.83 ± 0.04 | 0.03 | |
| Average distance (cm in 20 min) | 2777 ± 810 | 2386 ± 755 | 4467 ± 5870 | 3681 ± 3512 | ||
| TD-NMR | Body weight (g) | 27.4 ± 1.2 | 28.4 ± 1.7 | 23.8 ± 1.4 | 24.1 ± 1.8 | |
| Fat mass (g) | 5.7 ± 0.7 | 6.2 ± 0.9 | 5.5 ± 0.7 | 5.6 ± 0.9 | ||
| Lean mass (g) | 17.7 ± 0.6 | 18.1 ± 0.9 | 14.7 ± 0.8 | 14.9 ± 0.8 |
Analysis was done on 13-week-old mice under ad libitum conditions. n = 14–15 per genotype and sex. Data are presented as mean ± standard deviation. Significance vs. wild-type controls: Exact P values are indicated for P < 0.05
RER respiratory exchange ratio, TD-NMR time domain nuclear magnetic resonance