Fig. 6.

Large fragment deletion of the myxalamid biosynthetic gene cluster in M. xanthus DK1622 using the optimized spacer 17-6. a The seven sgRNA target sites (17-4 to 17-10) were revealed in or near the myxalamid gene cluster. The genes involved in the biosynthesis of myxalamid are shown in arrows. The positions of the guide sequence are indicated. b Identification of mutant strains by agarose gel electrophoresis of PCR product. The 4.0-kb positive fragment (shown with red arrow) appeared in different mutants (1–16), but not in DK1622 (c). c Chromatograms of the 4.0-kb PCR product DNA sequences, sequenced using the PCR primers as the sequencing primers. d EIC of myxalamid A ([M+H]⁺ m/z 416.31) from DK1622 (red) and mutant (blue)