Fig. 7.

Comparison of the yields of secondary metabolites in M. xanthus DK1622 and epothilone-producing mutant with the deletion of myxalamid or myxovirescin genes. a In each single strain, the yield of DKxanthene 534 was set as standard, and the ratios of the yields of other metabolites were provided. Experiments were performed in triplicate. Error bars indicate standard deviations. DK-dMA17, the myxalamid-deleted mutant from DK1622; DK-dMV11, the myxovirescin-deleted mutant from DK1622; KE10dD-MV, the myxovirescin-deleted mutant from the epothilone-producing strain KE10. b EIC of myxovirescin A ([M+H]⁺ m/z 624.44, red) and epothilone B ([M+H]⁺ m/z 508.27, green) in KE10Dp11t. c EIC of myxovirescin A ([M+H]⁺ m/z 624.44, red) and epothilone B ([M+H]⁺ m/z 508.27, green) from KE10dD-MV. d The fragmentation patterns of epothilone B produced in the KE10Dp11t and KE10dD-MV with the correct molecular weight minus the H⁺, Na⁺, K⁺ (epothilone B = 507)