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. 2017 Aug 16;18:625. doi: 10.1186/s12864-017-4041-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Validation of five new protein-coding genes. a Detection of the protein synthesis of new Xe proteins. Total protein extracts of Xe 85–10 containing pBRM-P (XCV_PG13), pBRM-P (XCV_PG17), pBRM-P (XCV_PG07), pBRM-P (XCV_PG02) or pBRM-P (XCV_PG06) grown in NYG were separated by 15% SDS PAGE and analyzed by immunoblotting using a c-Myc-specific antibody. b Gene organization of the XCV_PG02 locus. XCV0209 and XCV_PG02 are highlighted. Arrows represent possible translation start codons of XCV_PG02