Fig. 3.

Interactions of two transcription factors (PPARG and PPARD) and their direct target genes that control lipid metabolism in abdominal fat of juvenile HG and LG chickens. This gene interaction network (a) was identified by microarray analysis and Ingenuity® Pathway Analysis (IPA) from a combined unique list (647 AR-DE genes) merged from DE genes in the main effect of genotype (averaged across 6 ages) with DE genes from the interaction of age and genotype (see Fig. 2). Red gene symbols indicate higher expression in abdominal fat of HG birds, while green gene symbols indicate higher expression in the LG. Ingenuity® Upstream Regulator Analysis identified direct target genes (b) of two opposing ligand-activated transcription factors (PPARG and PPARD). IPA predicts the activation of PPARG (orange symbol and arrows) and inhibition of PPARD (blue symbols and arrows), based on the observed expression of DE genes and the expected mammalian responses found in the Ingenuity Knowledge Base