Fig. 9.

RNA-Seq analysis of abdominal fat in HG and LG cockerels at 7 wk. shows a gene interaction network centered on interactions between SREBP2 and the AR (a). SREBP2 and its direct targets are expressed higher in the HG cockerels, while the AR and 8 target genes were up-regulated in the LG. Ingenuity Upstream Analysis predicts that the AR should be activated in the HG (orange gene symbol), based on the up-regulated condition (orange arrows and red gene symbols) of 27 target genes (b). An additional 22 DE genes, known direct targets of the AR, were up-regulated (green gene symbols) in abdominal fat of the LG birds. Ingenuity predicted activation is indicated by orange arrows), while predicted inhibition is shown by blunted blue lines