Table 2.

IPA summary of microarray analysis of abdominal fat in HG and LG cockerels (1-11 wk)

Top Canonical Pathways	p-value	Overlap	Ratio
Oxidative Phosphorylation	7.88E-10	17.4%	19/109
Mitochondrial Dysfunction	1.37E-08	12.9%	22/171
EIF2 Signaling	2.33E-07	11.4%	21/185
NRF2-mediated Oxidative Stress Response	2.84E-06	10.6%	19/180
Phagosome maturation	7.93E-05	10.8%	13/120
Top Upstream Regulators	p-value of overlap		# Target genes
TP53	2.92E-21		105
PPARA	1.46E-18		50
MYC	2.75E-16		78
PPARG	1.94E-14		45
MYCN	2.51E-13		32
Top Molecular and Cellular Functions	p-value		# Genes
Cellular Growth and Proliferation	1.96E-04 - 1.11E-16		285
Cellular Movement	2.13E-04 - 3.09E-14		171
Lipid Metabolism	2.79E-04 - 7.14E-13		129
Molecular Transport	2.72E-04 - 7.14E-13		176
Small Molecule Biochemistry	2.79E-04 - 7.14E-13		163
Physiological System Development and Function	p-value		# Genes
Cardiovascular System	2.58E-04 - 7.81E-12		107
Organismal Development	2.58E-04 - 7.81E-12		181
Organismal Survival	4.90E-05 - 3.31E-10		175
Hematological System	2.72E-04 - 3.05E-08		123
Immune Cell Trafficking	2.72E-04 - 3.05E-08		72
Top Up-regulated genes	HG/LG Ratio	Top Down-regulated genes	HG/LG Ratio
FN1	12.58	TGM2	−5.86
SCD	10.69	MED27	−5.06
PPP1R9A	9.23	SESTD1	−4.07
XYLT2	5.14	ALB	−3.80
PIGC	4.52	RPL18A	−3.78
NFASC	4.02	MT-ND1	−3.21
PLEC	3.55	HSD11B1	−3.11
ABHD3	3.40	BM2	−3.00
FASN	3.34	TAGLN	−2.95
ARID4B	3.30	ANGPTL3	−2.94

Ingenuity Pathway Analysis (IPA) was used for functional annotation and mapping of 647 DE genes from the time-course microarray analysis of abdominal fat in HG and LG cockerels (1-11 wk) that were “Analysis Ready” (AR-DE). This unique (non-redundant) gene set was compiled from AR-DE genes found in the main effect of genotype or the genotype x age interaction