Table 4.
Differential gene expression in abdominal fat of HG and LG chickens across three analytical methods
| qRT-PCR Analysis | RNA-Seq Analysis | Microarray Analysis | ||||
|---|---|---|---|---|---|---|
| Gene Symbol | Fold-change | P-Value | Fold-change | P-Value | Fold-change | P-Value |
| ALB | −345.12 | 1.38E-10 | −169.4 | 5.00E-05 | −1.48 | 0.0100 |
| ALDOB | 2.75 | 0.0037 | −98.8 | 5.00E-05 | 1.4 | 0.0004 |
| A2M | −2.27 | 0.0412 | −52.7 | 5.00E-05 | −5.81 | 0.0009 |
| AGTR1 | −2.08 | 0.0035 | −1.93 | 6.63E-04 | −1.92 | 0.0579 |
| ANXA1 | 3.4 | 0.0002 | 2.29 | 5.00E-05 | 2.52 | 0.1303 |
| CEBPA | 1.73 | 0.0302 | 1.44 | 0.0008 | 3.19 | 0.0055 |
| F5 | −3.21 | 0.016 | −3.24 | 6.63E-04 | −4.34 | 0.1336 |
| EX-FABP | −41.12 | 1.22E-08 | −33.07 | 6.63E-04 | −5.78 | 0.0069 |
| FADS2 | 4.9 | 0.0003 | 4.08 | 5.00E-05 | 2.15 | 0.0004 |
| FASN | 2.78 | 0.0033 | 2.02 | 5.00E-05 | 1.35 | 0.0003 |
| FGA | −1929.39 | 8.46E-11 | −282.08 | 5.00E-05 | −7.91 | 0.0002 |
| HPGDS | −2.76 | 0.0003 | −4.57 | 5.00E-05 | −4.05 | 0.0033 |
| HSD17B7 | 3.38 | 2.21E-05 | 3.48 | 5.00E-05 | 5.15 | 0.0401 |
| LDHA | −2.74 | 0.015 | −2.02 | 5.00E-05 | −1.58 | 0.0484 |
| LPIN1 | 2.22 | 0.0278 | 1.47 | 0.0001 | 1.1 | 0.5185 |
| LPL | 3.69 | 0.0002 | 2.15 | 0.0032 | 1.54 | 0.0007 |
| ND6 | −1.78 | 0.037 | −2.55 | 5.00E-05 | −1.28 | 0.0309 |
| PPARG | 2.55 | 0.0047 | 1.70 | 5.00E-05 | 1.66 | 0.2626 |
| PYGL | 3.07 | 0.0068 | 2.14 | 0.0833 | 1.45 | 0.0052 |
| PLG | −7.22 | 0.0652 | −299.71 | 0.0001 | −3.52 | 0.0493 |
| PGRMC1 | 1.36 | 0.1159 | 1.7 | 1 | 2.42 | 0.0205 |
| PDK4 | −4.95 | 0.0056 | −6.14 | 5.00E-05 | −1.25 | 0.0002 |
| SCD | 89.12 | 0.002 | 129.4 | 5.00E-05 | 3.53 | 4.52E-13 |
| THBS2 | 3.57 | 0.0012 | 2.97 | 5.00E-05 | 5.18 | 2.33E-05 |
| TTR | −20.01 | 9.86E-07 | −35.72 | 5.00E-05 | −1.73 | 0.0229 |
| DHCR24 | 6.18 | 0.0001 | 5.46 | 5.00E-05 | - | - |
| ACACA | 1.85 | 0.056 | 1.82 | 5.00E-05 | - | - |
| ACE | 1.91 | 0.0269 | 2.47 | 6.63E-04 | - | - |
| AGT | −24.69 | 1.73E-06 | −39.29 | 6.63E-04 | - | - |
| APOH | −24.98 | 1.48E-06 | −122,00 | 5.00E-05 | - | - |
| CDS2 | 1.99 | 0.0729 | 1.81 | 6.63E-04 | - | - |
| F8 | −1.49 | 0.0589 | −2.26 | 6.63E-04 | - | - |
| DGKQ | 4 | 1.08E-05 | 3.18 | 5.00E-05 | - | - |
| DPP7 | 2.73 | 0.0003 | 2.69 | 0.0086 | - | - |
| FGB | −32.54 | 1.11E-06 | −324.53 | 5.00E-05 | - | - |
| FGG | −118.03 | 0.0016 | −197.44 | 5.00E-05 | - | - |
| FZD9 | 1.54 | 0.0201 | 2.07 | 6.63E-04 | - | - |
| GPD1 | 3.25 | 6.74E-06 | 1.92 | 5.00E-05 | - | - |
| GREM1 | 2.02 | 0.0007 | 3.41 | 5.00E-05 | - | - |
| IGFALS | 2.13 | 0.0287 | 2.49 | 5.00E-05 | - | - |
| KLF5 | −3.09 | 0.0076 | −2.70 | 5.00E-05 | - | - |
| OSBP2 | 2.1 | 0.0561 | 2.07 | 5.00E-05 | - | - |
| PDE1C | −1.84 | 0.0078 | −2.04 | 5.00E-05 | - | - |
| SELE | −3.28 | 0.0139 | −7.81 | 5.00E-05 | - | - |
| SELP | −2.08 | 0.0089 | −2.74 | 5.00E-05 | - | - |
| SERPINB2 | −6.11 | 0.0035 | −8.87 | 5.00E-05 | - | - |
Fold-change values [(+) is higher expression in HG and (−) is higher expression in LG chickens] provided across three independent transcriptional analysis methods at 7 wk. P-value for qRT-PCR and microarray analyses were determined by a Student’s T-test on expression values at 7 wk. P-value for RNA-Seq is corrected for FDR (FDR ≤ 0.05)