Fig. 7.

ANXA2 knockdown could enhance the chemotherapeutic response to doxorubicin in NB xenograft nude mice. a, b. The volume of tumors obtained from animal models were decreased after doxorubicin treatment in vivo, and in ANXA2 knockdown group by shRNA. Efficacy was more substantial compared with control. c, d. H&E staining of tumor samples of nude mice obviously showed appearance change following chemotherapy: decrease in numbers of small round NB cells (red arrow), more foci of hemorrhage and necrosis (blue arrow). Particularly in ANXA2 knockdown groups, more cytotoxic effect could be found: less cancer cells and greater degree of vacuolar formation (green pentagram) following chemotherapy. c, e~h. IHC staining showed apoptotic factor AIF and cleaved-PARP proteins were highly expressed in chemotherapy groups. Corresponding quantification of e~h was performed as previously reported of ANXA2 IHC grade in Fig. 2bc. Original widow is × 400, magnified window is ×800, Bar represents 50 μm, *: p < 0.05, **: p < 0.01, ***: p < 0.001, The plotted error bars represent mean ± SEM, All results are from at least three independent experiments