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. 2017 Aug 16;17:193. doi: 10.1186/s12862-017-1044-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Chromosomal distribution of the VSAREP satellite DNA (stDNA) isolated from each Australian varanids on a DAPI-stained metaphase spread prepared from three Australian varanid lizards: Varanus acanthurus (a – d), V. rosenbergi (e – h), and V. gouldii (i – j). Hybridization patterns of rhodamine-labeled VSAREP stDNA (red) ((V. acanthurus, clone no. 3: SFII) b, (V. rosenbergi, clone no. 14: SFII) f, and (V. gouldii, clone no. 13: SFI) j) or FITC-labeled VSAREP stDNA (green) ((V. acanthurus, clone no. 4: SFIII) c and (V. rosenbergi, clone no. 9: SFI) g) and their co-hybridization pattern (d, h). Fluorescent DAPI-stained pattern of chromosomes are shown in a, e, and i. Arrowheads indicate the hybridization signals. Scale bars represent 10 μm