Table 4. Single-base deletions generated by pol γ.
| No. of mutants
|
Error rate × 10-4
|
Fold difference
|
||
|---|---|---|---|---|
| dNTP pool | -1total | -1(C) | ||
| Exonuclease-deficient pol γ | ||||
| Equimolar (1 μM) | 10 | 1 | 0.19 | 1.0 |
| Heart (SSM) | 5 | 2 | 2.6 | 14 |
| Heart (IFM) | 10 | 5 | 3.5 | 18 |
| Skeletal muscle | 4 | 2 | 5.7 | 30 |
| Wild-type pol γ | ||||
| Equimolar (1 μM) | 0 | 0 | ≤2.5 | 1.0 |
| Heart (SSM) | 1 | 1 | 9.6 | ≥3.8 |
| Heart (IFM) | 1 | 1 | 8.8 | ≥3.5 |
| Skeletal muscle | 0 | 0 | — | — |
-1total no. of mutants, number of single-base deletions observed in the total number of mutants observed described in Table 3. The error rate of 2.5 × 10-4 for wild-type pol γ with equimolar dNTPs is the error rate calculated if one mutant had been observed.