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. 2017 Jul 5;8(11):2033–2041. doi: 10.7150/jca.18714

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EMT in INHBB knockdown cells. (A) Western blotting of E-cadherin, Zo-1, and Snail proteins in shINHBB cells are treated with/without activin B. Treatment of shINHBB cells with activin B shows decreased levels of E-cad and Zo-1 and increased levels of Snail compared with the control. (B) Brightfield and immunofluorescence images of shINHBB cells are treated with/without activin B. The shINHBB cells treated with activin B shows loss of E-cadherin on the cell membrane. Scale bars: 20 μm (left panels); 5 μm (middle and right panels).