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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: J Mol Biol. 2014 Jun 2;426(15):2827–2839. doi: 10.1016/j.jmb.2014.05.023

Fig. 1.

Fig. 1

Expression of a single-copy, formate-responsive fdhF‘-’lacZ transcriptional fusion in different E. coli strains grown anaerobically. Strains were grown in TGYEP rich medium supplemented with the formate concentrations indicated (see also Materials and Methods). Cultures were harvested for analysis of β-galactosidase enzyme activity in the mid-exponential phase of growth (OD600 nm between 0.6 and 0.8). The strains analyzed included (a) wild-type MC4100, (b) REK701 (focA), (c) 234M1 (pflA) (d) 234M1 containing plasmid pASK-IBA5focA and (e) RM220 (ΔpflB ΔpflA).