Figure 4.

IP₆ as a Structural Cofactor of Pds5B

(A) Zoomed in view of the IP₆-binding site of Pds5B. IP₆ is shown as stick, along with its 2Fo-Fc electron density map (blue mesh) contoured at 1.0 σ. IP₆-binding residues are shown as sticks and labeled.

(B) 1D ¹H NMR spectra of authentic IP₆ standard (top) and IP₆ isolated from recombinant human Pds5B, with the ¹H assignment indicated.

(C) Surface drawing of human Pds5B colored with its electrostatic potential (blue, positive; red, negative). IP₆ and the Wapl peptide are shown in sticks.

(D) Anti-Myc and anti-β-tubulin immunoblots of lysates of HeLa cells transfected with the same amount of the indicated Myc-Pds5B plasmids. WT, wild type (see also Figure S6).