Fig. 7.

Treatment with PKR inhibitor, but not pentamidine, blocks foci formation and apoptosis in DM2-106 retinae. (A-H) Shown are 42 h APF retinae from control (A), untreated GMR>DM2-106 (B) and GMR>DM2-106 treated with various buffers and drugs (C-H) as indicated. These retinae were labeled for CCUG foci (red) and nuclei (blue). Arrows indicate example foci, both nuclear and cytoplasmic. Whereas pentamidine treatment did not block foci formation up to a concentration of 350 μM, treatment with PKR inhibitor (oxindole/imidazole derivative C16, PKR-I) suppressed foci formation in a concentration-dependent manner. (I-L) Eye imaginal discs from control (I), untreated GMR>DM2-106 (J) and GMR>DM2-106 treated with the indicated concentrations of PKR-I (K,L). Eye discs were obtained from third instar larvae and were labeled by TUNEL as an apoptotic marker. Arrows indicate apoptosis in the GMR-expression area.