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. 2017 Aug 1;10(8):1015–1025. doi: 10.1242/dmm.028852

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Morphology of the sciatic nerve and secondary perineural adhesions 1 week after external neurolysis and glutaraldehyde glue removal. (A) Comparison of recurrence of perineural scar tissue demonstrated severe adhesions within the GG^high group (right bar), in contrast to the GG^low group (left bar); n=2. (B) Gross morphological analysis revealed the formation of connective tissue (upper image) and regrowth of strong fibrotic fibers (lower image, encircled) 1 week after removal of 50 µl applied glutaraldehyde glue. (C) Representative cross sections of sciatic nerves of GG^high (left column) and GG^low (right column) were stained with H&E, Masson's trichrome, anti-neurofilament protein, anti-S100 and Luxol fast blue (from top to bottom). Comparison of both experimental groups revealed major differences in growth of perineural collagen-rich connective tissue and intraneural damage. Scale bars: 50 µm (high magnification) and 200 µm (inset).