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A-C. Denaturing gels showing Polθ terminal transferase activity on the indicated ssDNA (A and B) and RNA (C) substrates in the presence of the indicated nucleotides. Lanes 1 lack Polθ and nucleotides. Lanes 2 and 3 include Polθ and the indicated nucleotides. ssDNA and RNA sequences are indicated at bottom. *, 32P radio-label.
