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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Nat Protoc. 2016 Apr 28;11(5):1007–1020. doi: 10.1038/nprot.2016.053

Figure 2.

Figure 2

Gating strategy for the isolation of specific cell populations via FACS. (a) Representative illustration of the gating for the isolation of distinct fractions of kidney marrow cells: erythroid (E), lymphoid (L), precursor (P), and myeloid (M) populations. (b) Schematic illustration of gating for the isolation of cd41:GFPmedium cells from the combined lymphoid and progenitor (L+P) scatter fractions of adult WKM. Contour plots represent results obtained from analysis of kidneys of five adult Tg(cd41:GFP) fish. Modified from ref. 17; originally published in Blood. Svoboda et al. Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Blood. 2014;124:220–228. © The American Society of Hematology.