Enumeration of colonies from fractionated WKM cells and cd41medium cells. Colonies were generated by cell culture in methylcellulose with Epo, Tpo, or Gcsf or with a combination of Epo and Tpo or of Epo, Tpo, and Gcsf. Overall cell differentiation potential is represented by the number of colony-forming units (CFUs) per 100,000 cells plated. Bars represent mean values of biological triplicate experiments, and error bars represent s.d. Modified from ref. 17; originally published in Blood. Svoboda et al. Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Blood. 2014;124:220–228. © The American Society of Hematology.