TABLE 1.
Cytokines added | Colony type detected | Suitable zebrafish strain for colony detection | Note |
---|---|---|---|
Epo | CFU-E, BFU-E | Tg(b-globin:GFP)41, Tg(gata1:dsRed)26 | |
Gcsfa/b | CFU-G, CFU-M | Tg(mpx:GFP)28, Tg(lyz:dsRed)42, Tg(mpeg1:GFP)43 | |
Tpo | CFU-T | Tg(cd41:GFP)27 | |
Epo, Gcsfa/b | CFU-GEM | Tg(gata1:dsRed, mpx:GFP)16 | |
Epo, Scfa | CFU-E, BFU-E | Tg(b-globin:GFP)41, Tg(gata1:dsRed)26 | Addition of Scfa increases the proliferation capacity of erythroid colonies |
Epo, Tpo | CFU-E, BFU-E, CFU-T, CFU-TE | Tg(gata1:dsRed; cd41:GFP)18 | |
Epo, Gcsfa/b, Tpo | CFU-E, BFU-E, CFU-G, CFU-M, CFU-T, CFU-TE, CFU-GEM, CFU-GEMT | Tg(lyz:dsRed; cd41:GFP)18 | Erythroid colonies can be detected on the basis of hemoglobinization |
CFU-E, colony-forming unit erythroid; BFU-E, burst-forming unit erythroid; CFU-G, colony-forming unit granulocyte; CFU-M, colony-forming unit macrophage; CFU-T, colony-forming unit thrombocyte; CFU-TE, colony-forming unit thrombocyte, erythroid; CFU-GEM, colony-forming unit granulocyte, erythroid, macrophage; CFU-GEMT, colony-forming unit granulocyte, erythroid, macrophage, thrombocyte.