. Author manuscript; available in PMC: 2017 Aug 17.

Published in final edited form as: Nat Protoc. 2016 Apr 28;11(5):1007–1020. doi: 10.1038/nprot.2016.053

TABLE 1.

Colony generation depends on zebrafish cytokines present in the medium.

Cytokines added	Colony type detected	Suitable zebrafish strain for colony detection	Note
Epo	CFU-E, BFU-E	Tg(b-globin:GFP)⁴¹, Tg(gata1:dsRed)²⁶
Gcsfa/b	CFU-G, CFU-M	Tg(mpx:GFP)²⁸, Tg(lyz:dsRed)⁴², Tg(mpeg1:GFP)⁴³
Tpo	CFU-T	Tg(cd41:GFP)²⁷
Epo, Gcsfa/b	CFU-GEM	Tg(gata1:dsRed, mpx:GFP)¹⁶
Epo, Scfa	CFU-E, BFU-E	Tg(b-globin:GFP)⁴¹, Tg(gata1:dsRed)²⁶	Addition of Scfa increases the proliferation capacity of erythroid colonies
Epo, Tpo	CFU-E, BFU-E, CFU-T, CFU-TE	Tg(gata1:dsRed; cd41:GFP)¹⁸
Epo, Gcsfa/b, Tpo	CFU-E, BFU-E, CFU-G, CFU-M, CFU-T, CFU-TE, CFU-GEM, CFU-GEMT	Tg(lyz:dsRed; cd41:GFP)¹⁸	Erythroid colonies can be detected on the basis of hemoglobinization

CFU-E, colony-forming unit erythroid; BFU-E, burst-forming unit erythroid; CFU-G, colony-forming unit granulocyte; CFU-M, colony-forming unit macrophage; CFU-T, colony-forming unit thrombocyte; CFU-TE, colony-forming unit thrombocyte, erythroid; CFU-GEM, colony-forming unit granulocyte, erythroid, macrophage; CFU-GEMT, colony-forming unit granulocyte, erythroid, macrophage, thrombocyte.