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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Nat Protoc. 2016 Apr 28;11(5):1007–1020. doi: 10.1038/nprot.2016.053

TABLE 2.

Recommended methylcellulose volume and seeding density for selected multiwell plates.

Type of plate Methylcellulose volume per well (ml) Number of seeded cells per well

Fractionated WKM cells Sorted cd41medium HSPCs from lymphoid and progenitor scatter fraction
6-Well plate 3 40,000 4,000
12-Well plate 1.6 20,000 2,000
24-Well plate 0.9 10,000 1,000