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Recommended methylcellulose volume and seeding density for selected multiwell plates.
| Type of plate | Methylcellulose volume per well (ml) | Number of seeded cells per well | |
|---|---|---|---|
|
| |||
| Fractionated WKM cells | Sorted cd41medium HSPCs from lymphoid and progenitor scatter fraction | ||
| 6-Well plate | 3 | 40,000 | 4,000 |
| 12-Well plate | 1.6 | 20,000 | 2,000 |
| 24-Well plate | 0.9 | 10,000 | 1,000 |
