TABLE 3.
Primer sequences.
| Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Product size (bp) | Note |
|---|---|---|---|---|
| cd41 (ref. 44) | CTGAAGGCAGTAACGTCAAC | TCCTTCTTCTGACCACACAC | 197 | Thrombocytic, HSPC marker |
| c-mpl44 | CGCCAACCAAAGCCAGAGTTA | ACTTTTCAACAGGTGCATCCCA | 103 | Thrombocytic, HSPC marker |
| c-myb | GAGCTGTTCCGAACTCCCAA | TTAATCGTGCCGACCACTCC | 161 | HSPC marker |
| ccr9 (ref. 45) | AACCTCACTCACTCCTCAAAC | CAGACCACCAGAGTGTTACC | 189 | T cell, eosinophilic marker |
| csf1r46 | ATGACCATACCCAACTTTCC- | AGTTTGTTGGTCTGGATGTG | 148 | Macrophage marker |
| csf3r45 | TGAAGGATCTTCAACCACAC | GGGAATTATAGGCCACAAAC | 233 | Granulocytic marker |
| ef1a44 | GAGAAGTTCGAGAAGGAAGC | CGTAGTATTTGCTGGTCTCG | 142 | Housekeeping gene |
| fli1 (ref. 18) | CCGAGGTCCTGCTCTCACAT | GGGACTGGTCAGCGTGAGAT | 87 | Lymphoid, thrombocytic marker |
| gata1 (ref. 44) | TGAATGTGTGAATTGTGGTG | ATTGCGTCTCCATAGTGTTG | 211 | Erythroid marker |
| gata2 (ref. 47) | CCTGCGGGCTCTACTACAAACT | GTCTTGTCCTGCATGCACTTG | 160 | Endothelial, HSC, eosinophilic marker |
| hbA48 | CTGATACGGACAAGGCTGTTGT | AGACGGTCAGCATTCTGGCGA | 99 | Marker of adult erythrocytes |
| hbB48 | ATGGTTGAGTGGACAGATGC | TACACGATCAGACATCTGGATA | 107 | Marker of adult erythrocytes |
| hbBe3 (ref. 47) | TTTCCGGCTGTTAGCGGACT | TTGCCTTCTGAGGGCTGACA | 127 | Marker of primitive erythrocytes |
| lyz | CTGGTGGGAAGAATTTGTG | CCGTCCATTTTCACAATCAG | 100 | Neutrophilic marker |
| marco | ACGACAGCTTCGATAATTTG | AAAATACTGCTCTCGGTTCC | 145 | Macrophage marker |
| mhc2dab45 | CAGGCCTACTTGCATCAATTG | CAGACCAGATGCTCCGATG | 429 | B cell, macrophage, dendritic cell marker |
| mpeg1 (ref. 46) | CCCACCAAGTGAAAGAGG | GTGTTTGATTGTTTTCAATGG | 150 | Macrophage marker |
| mpx44 | TGATGTTTGGTTAGGAGGTG | GAGCTGTTTTCTGTTTGGTG | 161 | Neutrophilic marker |
| pax5 | CTGATTACAAACGCCAAAAC | CTAAATTATGCGCAGAAACG | 177 | B cell marker |
| pu.1 (ref. 44) | AGAGAGGGTAACCTGGACT | AAGTCCACTGGATGAATGTG | 204 | Myeloid marker |
| runx1 (ref. 44) | CGGTGAACGGTTAATATGAC | CTTTTCATCACGGTTTATGC | 139 | HSPC marker |
| tcra | TCGTTTTCAATGTGCTGGTG | GATGATCTGGAATGGGATGC | 139 | T cell marker |
Annealing temperature of listed primers is 60 °C. HSC, hematopoietic stem cell; HSPC, hematopoietic stem and progenitor cell.