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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Nat Protoc. 2016 Apr 28;11(5):1007–1020. doi: 10.1038/nprot.2016.053

TABLE 3.

Primer sequences.

Gene Forward primer sequence (5′-3′) Reverse primer sequence (5′-3′) Product size (bp) Note
cd41 (ref. 44) CTGAAGGCAGTAACGTCAAC TCCTTCTTCTGACCACACAC 197 Thrombocytic, HSPC marker
c-mpl44 CGCCAACCAAAGCCAGAGTTA ACTTTTCAACAGGTGCATCCCA 103 Thrombocytic, HSPC marker
c-myb GAGCTGTTCCGAACTCCCAA TTAATCGTGCCGACCACTCC 161 HSPC marker
ccr9 (ref. 45) AACCTCACTCACTCCTCAAAC CAGACCACCAGAGTGTTACC 189 T cell, eosinophilic marker
csf1r46 ATGACCATACCCAACTTTCC- AGTTTGTTGGTCTGGATGTG 148 Macrophage marker
csf3r45 TGAAGGATCTTCAACCACAC GGGAATTATAGGCCACAAAC 233 Granulocytic marker
ef1a44 GAGAAGTTCGAGAAGGAAGC CGTAGTATTTGCTGGTCTCG 142 Housekeeping gene
fli1 (ref. 18) CCGAGGTCCTGCTCTCACAT GGGACTGGTCAGCGTGAGAT 87 Lymphoid, thrombocytic marker
gata1 (ref. 44) TGAATGTGTGAATTGTGGTG ATTGCGTCTCCATAGTGTTG 211 Erythroid marker
gata2 (ref. 47) CCTGCGGGCTCTACTACAAACT GTCTTGTCCTGCATGCACTTG 160 Endothelial, HSC, eosinophilic marker
hbA48 CTGATACGGACAAGGCTGTTGT AGACGGTCAGCATTCTGGCGA 99 Marker of adult erythrocytes
hbB48 ATGGTTGAGTGGACAGATGC TACACGATCAGACATCTGGATA 107 Marker of adult erythrocytes
hbBe3 (ref. 47) TTTCCGGCTGTTAGCGGACT TTGCCTTCTGAGGGCTGACA 127 Marker of primitive erythrocytes
lyz CTGGTGGGAAGAATTTGTG CCGTCCATTTTCACAATCAG 100 Neutrophilic marker
marco ACGACAGCTTCGATAATTTG AAAATACTGCTCTCGGTTCC 145 Macrophage marker
mhc2dab45 CAGGCCTACTTGCATCAATTG CAGACCAGATGCTCCGATG 429 B cell, macrophage, dendritic cell marker
mpeg1 (ref. 46) CCCACCAAGTGAAAGAGG GTGTTTGATTGTTTTCAATGG 150 Macrophage marker
mpx44 TGATGTTTGGTTAGGAGGTG GAGCTGTTTTCTGTTTGGTG 161 Neutrophilic marker
pax5 CTGATTACAAACGCCAAAAC CTAAATTATGCGCAGAAACG 177 B cell marker
pu.1 (ref. 44) AGAGAGGGTAACCTGGACT AAGTCCACTGGATGAATGTG 204 Myeloid marker
runx1 (ref. 44) CGGTGAACGGTTAATATGAC CTTTTCATCACGGTTTATGC 139 HSPC marker
tcra TCGTTTTCAATGTGCTGGTG GATGATCTGGAATGGGATGC 139 T cell marker

Annealing temperature of listed primers is 60 °C. HSC, hematopoietic stem cell; HSPC, hematopoietic stem and progenitor cell.