Components	Volume	Final concentration
Methylcellulose stock	60 ml	1.2% (wt/vol)
H2O	16.3 ml
FBS	10 ml	10% (vol/vol)
Carp serum	2 ml	2% (vol/vol)
10% BSA	5 ml	0.5% (wt/vol)
5.6% NaHCO3	3.6 ml	0.2% (wt/vol)
Fe-SIH, 1,000×	0.1 ml
1 M 2-mercaptoethanol	10 μl	100 μM
Penicillin–streptomycin, 100×	1 ml	Penicillin (100 U/ml), streptomycin (100 μg/ml)
0.2 M L-glutamine	2 ml	4 mM