Skip to main content
. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Nat Protoc. 2016 Apr 28;11(5):1007–1020. doi: 10.1038/nprot.2016.053
Components Volume Final concentration
Methylcellulose stock 60 ml 1.2% (wt/vol)
H2O 16.3 ml
FBS 10 ml 10% (vol/vol)
Carp serum 2 ml 2% (vol/vol)
10% BSA 5 ml 0.5% (wt/vol)
5.6% NaHCO3 3.6 ml 0.2% (wt/vol)
Fe-SIH, 1,000× 0.1 ml
1 M 2-mercaptoethanol 10 μl 100 μM
Penicillin–streptomycin, 100× 1 ml Penicillin (100 U/ml), streptomycin (100 μg/ml)
0.2 M L-glutamine 2 ml 4 mM