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. 2017 Jul 21;8(12):2346–2355. doi: 10.7150/jca.19465

Figure 4.

Figure 4

Expression of CHI3L1, MMP13 and SPP1 mRNA or protein in ESCC cell lines and tissues and their locations in tissue. The mRNA and protein expression levels in six pairs of matched ESCC and noncancerous tissues was analyzed by real-time PCR and Western blotting, respectively (A, B), and in an immortalized esophageal epithelial cell line (NE-3) and esophageal carcinoma cell lines by real-time PCR and ELISA, respectively (C, D). The expression level was normalized to the expression of GAPDH and α-tubulin, respectively. Error bars represent standard deviations (SD) calculated from three parallel experiments. The locations of CHI3L1, MMP13 and SPP1 were determined by immunohistochemistry (E). The normal esophageal epithelial tissue showed no expression of CHI3L1, MMP13 or SPP1, respectively (E a, e, i, 200 × and b, f, j, 400×). The ESCC tissues exhibited high expression levels of these three biomarkers (E c, f, k, 200 × and d, h, l, 400×).