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. 2017 Jul 21;8(12):2356–2368. doi: 10.7150/jca.19470

Figure 5.

Figure 5

miR-204-5p overexpression or FOXC1 knockdown inhibits LSCC cell migration, invasion, and the EMT process. Scratch assay of cell migration by Hep-2 and TU-177 cells transfected with miR-204-5p mimics (50 nM) (A, B) or FOXC1 siRNA (50 nM) (C, D) for 12, 24, and 48 h. Relative number of invaded cells in a Transwell assay of Hep-2 and TU-177 cells transfected with miR-204-5p mimics (50 nM) (E, F) or FOXC1-siRNA (50 nM) (G, H). Hep-2 and TU-177 cells were transfected with miR-204-5p mimics (50 nM) or FOXC1-siRNA for 48 h, and expression of EMT markers E-cadherin, N-cadherin, and MMP-9 in Hep-2 cells (I) and TU-177 cells (J) was detected by immunoblotting. Data are presented as the mean ± SD.