Table 1.
The LAMP primers of Plasmodium unique genes.
| Gene ID. | Target region | LAMP primer | ||||
|---|---|---|---|---|---|---|
| Name | Start to end | Length (bp) | Name | Oligo (nt) | Sequence (5′-3′) | |
| PF3D7_0202200 | PF3D7_0202200-1 | 921-1121 | 201 | F3 | 18 | GAACAAGTTTCAAAAGCA |
| B3 | 18 | GTCATCTTGTTCCCTAAG | ||||
| FIP | 48 | CATACGTTTTTGCATATCCATATTACCTTTTATATTACCTTTCGTTCC | ||||
| BIP | 39 | TTCATGCTATGTTTAAATGGGC-CTTCATTTCTGCGTTGT | ||||
| PF3D7_0202200-2 | 604-798 | 195 | F3 | 17 | TTGGCGCTATAAGAGAA | |
| B3 | 20 | GTTTATCTTTTCCTTCATCA | ||||
| FIP | 40 | AGTTATACTTGCAGGTAATGGAAATAACGATTCCTTTGGT | ||||
| BIP | 46 | AAGGAGATTCTGAAGCAGAAGTATCATCATTTGAAGAACTTACAGC | ||||
| PF3D7_0202200-3 | 927-1121 | 195 | F3 | 19 | GTTTCAAAAGCATTACCTT | |
| B3 | 18 | GTCATCTTGTTCCCTAAG | ||||
| FIP | 38 | AACATTACATACGTTTTTGCATTACCTTTCGTTCCTCC | ||||
| BIP | 37 | TTCATGCTATGTTTAAATGGCTTCATTTCTGCGTTGT | ||||
| PF3D7_0202200-4 | 597- 798 | 202 | F3 | 17 | AAAAGAATTGGCGCTAT | |
| B3 | 20 | GTTTATCTTTTCCTTCATCA | ||||
| FIP | 45 | TGCAGGTAATGGTAAATGATTTGAGAAATGATAAATAACGATTCC | ||||
| BIP | 41 | ATAACTAAAGGAGATTCTGAAGATTTGAAGAACTTACAGCT | ||||
| PF3D7_0202200-5 | 660-883 | 224 | F3 | 18 | CATTTACCATTACCTGCA | |
| B3 | 20 | TGTAAATTCGATTTACCTGA | ||||
| FIP | 45 | TGATGTATTACGTGAATCATAGTATAACTAAAGGAGATTCTGAAG | ||||
| BIP | 42 | TGAAGGAAAAGATAAACGTCCACATATTTTTGATAATCCCAT | ||||
| PF3D7_0702300 | PF3D7_0702300-1 | 338-543 | 206 | F3 | 20 | ATCAGAATATCAATCGAACT |
| B3 | 19 | TTACTTTTGTAGTGCTTGT | ||||
| FIP | 34 | TGTTCCGGATTTTTTACTATTCTTGGTGGGGGAT | ||||
| BIP | 44 | TATACCAGAAAGTAGTAGTACAGTTGTACTGTTATTTGCTGCTA | ||||
| PF3D7_0702300-2 | 305–544 | 240 | F3 | 20 | ATCAACATACGTTATAAAGC | |
| B3 | 19 | GTTACTTTTGTAGTGCTTG | ||||
| FIP | 43 | GTTCCGGATTTTTTACTATTTACATATCAATCGAACTTTCTTG | ||||
| BIP | 44 | TATACCAGAAAGTAGTAGTACAGTTGTACTGTTATTTGCTGCTA | ||||
| PF3D7_0702300-3 | 347–543 | 197 | F3 | 18 | TCAATCGAACTTTCTTGG | |
| B3 | 19 | TTACTTTTGTAGTGCTTGT | ||||
| FIP | 41 | ACTTTGTATTTACATTTGTTCCGGAGGGATATAGTGCAGCT | ||||
| BIP | 41 | TATACCAGAAAGTAGTAGTACAGTTGTACTGTTATTTGCTG | ||||
| PF3D7_1001900 | PF3D7_1001900-1 | 691–873 | 183 | F3 | 16 | CGGAAGAAGGTGAAGA |
| B3 | 18 | TCAAAAGTATTGCTGTCA | ||||
| FIP | 44 | CCAATTTCTCCTTTATTTGGATATACCTAAACCTTATACAGTTG | ||||
| BIP | 45 | TTCGTAGAAGTGTTATTACTTTGTAATAAGAAAAGTAGGTGACGT | ||||
| PF3D7_1001900-2 | 681–895 | 215 | F3 | 17 | GTTGAAAATGCGGAAGA | |
| B3 | 20 | GCTGTCTTAAAATTGTCATT | ||||
| FIP | 47 | CCAATTTCTCCTTTATTTGGATTAGGTGAAGAAATACCTAAACCTTA | ||||
| BIP | 43 | TTCGTAGAAGTGTTATTACTGTCATTGTAATAAGAAAAGTAGG | ||||
| PF3D7_1002000 | PF3D7_1002000-1 | 707–930 | 224 | F3 | 18 | AATCAAATGTGACAAACG |
| B3 | 19 | GGTCTTTCTCTTTTTCCTT | ||||
| FIP | 48 | GTCTGCTTTTATTTCTTCAATTGAAAAGTGTGAAATTTGAAGAACCAC | ||||
| BIP | 43 | TAAACGTGGATGAAAAAAAGGTGTTTTTTCTTCTTCACTGGAA | ||||
| PF3D7_1002000-2 | 720–930 | 211 | F3 | 17 | AAACGTGAAAAGTGTGA | |
| B3 | 19 | GGTCTTTCTCTTTTTCCTT | ||||
| FIP | 39 | TGTCTGCTTTTATTTCTTCAAATTTGAAGAACCACAAGA | ||||
| BIP | 43 | TAAACGTGGATGAAAAAAAGGTGTTTTTTCTTCTTCACTGGAA | ||||
| PF3D7_1002000-3 | 666–892 | 227 | F3 | 19 | TACCTATTCTAGACGAGAA | |
| B3 | 19 | TTTTTTCTTCTTCACTGGA | ||||
| FIP | 41 | CTCATCTTGTGGTTCTTCTAGTAAATCAAATGTGACAAACG | ||||
| BIP | 39 | AAGCAGACAATAATGATTCAAATCCTTTTTTTCATCCAC | ||||
| PF3D7_1002000-4 | 744–969 | 226 | F3 | 18 | AGAACCACAAGATGAGAA | |
| B3 | 20 | ATAAGATGTGTTACTGTCTT | ||||
| FIP | 43 | TCATCCACGTTTATATTAGTCAATTGAAGAAATAAAAGCAGAC | ||||
| BIP | 42 | AGTGTAATTTCCAGTGAAGAAGATTCTTATGAGACCTTTTGG | ||||
| PF3D7_1148900 | PF3D7_1148900-1 | 48–267 | 220 | F3 | 17 | CGTGAAAACATTGATGA |
| B3 | 18 | CGTTACATAAAATGCTGA | ||||
| FIP | 35 | CTTTGCCAAAAACTTTCAGAAAATTCCTTTCAAGG | ||||
| BIP | 39 | TTCTGCCGTTGTTTTATTTTTTGACTAAAAGTGCTTTGA | ||||
| PF3D7_1148900-2 | 56–267 | 212 | F3 | 19 | CATTGATGAAGAAAATTCC | |
| B3 | 18 | CGTTACATAAAATGCTGA | ||||
| FIP | 28 | GAAGGGCTTTGCCTTTCAAGGGTCCAAG | ||||
| BIP | 39 | TTCTGCCGTTGTTTTATTTTTTGACTAAAAGTGCTTTGA | ||||
| PF3D7_1240100 | PF3D7_1240100-1 | 25–236 | 212 | F3 | 20 | ATTTTATATCTCATTGCTGC |
| B3 | 18 | AGTCCTATACCATATGCA | ||||
| FIP | 48 | CCTTTCTTTTTTTTTTTTCCCTC-TTGGCAATTAATTTAATAGCTCCCA | ||||
| BIP | 42 | TTCAGAAAAAAACATCATAAGGCCAAATAAAAGAGCGATAGC | ||||
| PF3D7_1240100-2 | 41–228 | 188 | F3 | 17 | CTGCCTTATTGGCAATT | |
| B3 | 17 | ACCATATGCAGTACCAA | ||||
| FIP | 42 | GAGGACATCCTTTCTTTTTTTTTATTTAATAGCTCCCAGTGT | ||||
| BIP | 47 | TTCAGAAAAAAACATCATAAGGCTAAAAGAGCGATAGCAGAAACAAC | ||||
| PF3D7_1240100-3 | 41- 228 | 188 | F3 | 17 | CTGCCTTATTGGCAATT | |
| B3 | 17 | ACCATATGCAGTACCAA | ||||
| FIP | 34 | AGAGGACATCCTTTCTTTCCCAGTGTTTGTAACG | ||||
| BIP | 47 | TTCAGAAAAAAACATCATAAGGCTAAAAGAGCGATAGCAGAAACAAC | ||||
| PF3D7_1253300 | PF3D7_1253300-1 | 359–570 | 212 | F3 | 17 | TTGACAAGAGAGGAGTT |
| B3 | 18 | CCATGTGATTTTAGGAGG | ||||
| FIP | 45 | ACCATTTTGTGATTCCATATTTGATTTATTAGTTCAAGTACCACC | ||||
| BIP | 42 | ATGTAGACAAGGAACAAAGGATTTAATTTGGGTTACCCGTAT | ||||
| PF3D7_1253300-2 | 328–532 | 205 | F3 | 18 | AGATAGCAAATTCGAGAG | |
| B3 | 16 | GGTTACCCGTATGTCT | ||||
| FIP | 41 | GTGGTACTTGAACTAATAAATCACGTAACTGAACAGTTGAC | ||||
| BIP | 35 | TATGGAATCACAAAATGGTAGGTGCAATTCCTCTT | ||||
| PF3D7_1253300-3 | 542–738 | 197 | F3 | 18 | ATGGTAAATCACCTCCTA | |
| B3 | 21 | CTTCACATATAAATCGGTATG | ||||
| FIP | 38 | TGTTTCCAAAGTAGAAACCAAATCACATGGCAAGGATG | ||||
| BIP | 45 | ATAACCTACTTAATAAAAAGGCTCCCATCTATACATGAACGAATG | ||||
| PF3D7_1253300-4 | 521–738 | 218 | F3 | 17 | ATACGGGTAACCCAAAT | |
| B3 | 21 | CTTCACATATAAATCGGTATG | ||||
| FIP | 43 | CAAAGTAGAAACCATCATATTACTCACCTCCTAAAATCACATG | ||||
| BIP | 42 | ACCTACTTAATAAAAAGGCTCTATACATGAACGAATGAAACT | ||||
| PF3D7_1253300-5 | 507–703 | 197 | F3 | 20 | ACTTACATATAGACATACGG | |
| B3 | 20 | CATCTATACATGAACGAATG | ||||
| FIP | 45 | AATCATACGAACATCCTTGCACCCAAATCAATATGGTAAATCACC | ||||
| BIP | 47 | ATATGATGGTTTCTACTTTGGATGGAGCCTTTTTATTAAGTAGGTTA | ||||
| PF3D7_1301700 | PF3D7_1301700-1 | 688–913 | 226 | F3 | 21 | TCAATGCACTCAAATCACAAT |
| B3 | 21 | ACATATAACATAGCTGGGACA | ||||
| FIP | 45 | TGCAGCTACTGCTGAAATAGTTAATTAATGTTAGAGCAGCTACCA | ||||
| BIP | 46 | ATATGCTTGCTATTGCAGGAGTTAATAATAATGCCATACCAGGTAA | ||||
| PF3D7_1301700-2 | 722–913 | 192 | F3 | 19 | TGTTAGAGCAGCTACCATT | |
| B3 | 21 | ACATATAACATAGCTGGGACA | ||||
| FIP | 46 | ATGTTGCAGCTACTGCTGAAAATAGCAGGATTCTTATCAATCTTTG | ||||
| BIP | 46 | ATATGCTTGCTATTGCAGGAGTTAATAATAATGCCATACCAGGTAA | ||||
| PF3D7_1334700 | PF3D7_1334700-1 | 351–553 | 203 | F3 | 20 | ACAAGAATGTCAATCAATCA |
| B3 | 17 | TTTGGCTAGTTCCTCTT | ||||
| FIP | 46 | GTAGAGAAAGGAGTATTAACATTATAAAAGAACTTTTGAGTGCTGC | ||||
| BIP | 40 | GCATTAGAAAAAGATGAATTCCTGTATGTCCACATAGTGC | ||||
| PF3D7_1334700-2 | 235–441 | 207 | F3 | 19 | AGTTTATTAGACCAACCAA | |
| B3 | 19 | AGTTGTAGAGAAAGGAGTA | ||||
| FIP | 40 | GGATCATTTGGATTTGTTAAATTTCTACATCAACACAAGG | ||||
| BIP | 42 | CAAGAATGTCAATCAATCAATGTTCATCATTTGTAGCAGCAC | ||||
| PF3D7_1334700-3 | 211–428 | 218 | F3 | 17 | AATTCCCTTGAAGAAGC | |
| B3 | 21 | GGAGTATTAACATGTTCATCA | ||||
| FIP | 48 | GAAGTATTATTTTCACTACCTTGTGACAAAAGAGTTTATTAGACCAAC | ||||
| BIP | 41 | TAACAAATCCAAATGATCCAACACCAGCACTCAAAAGTTCT | ||||
| PF3D7_1334700-4 | 247–441 | 194 | F3 | 23 | CAACCAAATATTTCTACATCAAC | |
| B3 | 19 | AGTTGTAGAGAAAGGAGTA | ||||
| FIP | 45 | GGATCATTTGGATTTGTTAACAAGGTAGTGAAAATAATACTTCAG | ||||
| BIP | 42 | CAAGAATGTCAATCAATCAATGTTCATCATTTGTAGCAGCAC | ||||
| PF3D7_1334700-5 | 278–509 | 232 | F3 | 23 | GTGAAAATAATACTTCAGGTGTT | |
| B3 | 18 | CCACATAGTGCTTCTGTT | ||||
| FIP | 45 | GCAGCACTCAAAAGTTCTTTTATAATAACAAATCCAAATGATCCA | ||||
| BIP | 41 | ATGATGAACATGTTAATACTCCCGTGATACTATCACGGAAT | ||||
| PF3D7_0112300 | 18S rRNA | 506-725 | 220 | F3 | 24 | TGTAATTGGAATGATAGGAATTTA |
| B3 | 23 | GAAAACCTTATTTTGAACAAAGC | ||||
| FIP | 41 | AGCTGGAATTACCGCGGCTGGGTTCCTAGAGAAACAATTGG | ||||
| BIP | 45 | TGTTGCAGTTAAAACGTTCGTAGCCCAAACCAGTTTAAATGAAAC | ||||