Figure 12.

Effects of C8 substituents on moxifloxacin-induced DNA cleavage mediated by wild-type gyrase and quinolone-resistant mutants. The abilities of low (left) or high (right) concentrations ranges of 8-H-moxifloxacin (top) and 8-methyl-moxifloxacin (bottom) to enhance cleavage mediated by wild-type (black), S85L (blue), and E89K (red) gyrase were determined. Cleavage induced by moxifloxacin with wild-type gyrase is shown by the dotted black line in the top left panel. Drug structures are shown in the right panels. Error bars represent the standard deviation of at least three independent experiments.