Figure 6.

Ability of cipro-dione and moxi-dione to compete with 3′-(AM)P-dione in DNA cleavage reactions mediated by wild-type gyrase. The abilities of increasing concentrations of cipro-dione (black) and moxi-dione (red) to compete with and prevent cleavage enhancement by 5 μM 3′-(AM)P-dione were determined. Drugs were added simultaneously to reaction mixtures. The low levels of cleavage seen in the presence of cipro-dione or moxi-dione alone were subtracted from the levels of cleavage seen with both drugs, and the resulting corrected cleavage level is plotted relative to the level observed with 3′-(AM)P-dione alone (set to 1.0). Error bars represent the standard deviation of at least three independent experiments.