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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Biomaterials. 2017 Mar 26;130:1–13. doi: 10.1016/j.biomaterials.2017.03.024

Figure 8. Delivery of CEH plasmid via Gal-G5 results in increased CEH expression and associated biological activity.

Figure 8

(A) CEH expression enhanced by Gal-G/CEH. Primary mouse hepatocytes were treated with G5/pCMV (4:1, w/w) (control), G5/CEH (4:1: w/w), Gal-G5/pCMV (20:1, w/w) (control) or Gal-G5/CEH (20:1, w/w) for 24 h. Following replacement with fresh growth medium, the hepatocytes were incubated for an additional 24 or 48 h. At the end of the treatment, total RNA was isolated and CEH gene expression was determined by using RT-qPCR. CEH expression induced by G5/CEH or Gal-G5/CEH relative to that induced by the corresponding control is presented. n=3, *P<0.05 and **P<0.01. (B) Intracellular hydrolysis of HDL-CE promoted by Gal-G5/CEH. Primary mouse hepatocytes were incubated with G5, Gal-G5, G5/CEH (4:1, w/w), or Gal-G5/CEH (20:1, w/w) for 24 h. Then the hydrolysis of HDL-3H-CE in each group was monitored. n=3, *P<0.05. (C) The flux of FC to bile acids as a result of CEH-mediated hydrolysis of HDL-3H-CE was assessed by monitoring the appearance of [3H]-BA in the culture medium. n=3, *P<0.05.