Figure 3.

Characteristics of the spontaneous seizures recorded during a 4‐week‐long video‐EEG recording period in DBA/2J mice following exposure to repeated flurothyl‐induced seizure. (A) Average and total number of spontaneous seizures that occurred during the 4‐week recording period in DBA/2J mice exposed to flurothyl as compared to controls grouped according to weeks. Significant differences exist between flurothyl‐exposed and control mice (week 1: p < 0.004; week 2: p < 0.009; week 3: p < 0.004; week 4: p < 0.02). The x axis represents the duration of the recording period in weeks, whereas the y axis represents both the average and SEM, and individual number of seizures expressed by the DBA/2J mice exposed to flurothyl (red circles). (B) Percentage of DBA/2J mice expressing spontaneous seizures during the 4‐week recording period binned by weeks. Significant differences were observed between flurothyl‐exposed and control mice (Fisher exact tests: week 1: p < 0.004; week 2: p = 0.05; week 3: p < 0.02; week 4: p = 0.05). (C) Circadian pattern of seizure distribution in DBA/2J mice during the 4‐week recording period grouped by weeks. Significant differences exist between day and night only during week 2 of the recording period (p < 0.05). Seven a.m. to 7 p.m. represents day (the animals’ normal sleep cycle) during which the lights were turned on in the animal facility. (D) Average duration of spontaneous seizures in DBA/2J mice across the 4‐week recording period. The duration of these seizure events was significantly different only between weeks 1 and 3 of the recording period (p < 0.002).