Fig. 3.
Real-time PCR (RT-PCR) was used to validate the microarray results for PBMC data sets (TaqMan PCR detection, Applied Biosystems). Probes and primers for 12 target sequences were designed using Primer Express computer software (Applied Biosystems). Data are presented as median values [±95% confidence limit (CL)]; n = 4. CD8A, CD8 antigen, α-polypeptide (p32); CD3G, CD3G antigen, γ-polypeptide (TiT3 complex); SH2D1A, SH2 domain protein 1A; TRB, T cell receptor-β locus; IL7R, interleukin (IL)-7 receptor; LAT, linker for activation of T cells; CSFR2, colony-stimulating factor 2 receptor-β, low affinity; FAS, tumor necrosis factor receptor superfamily, member 6; SOD2, superoxide dismutase 2, mitochondrial; ADM, adrenomedullin; S100A12, S100 calcium-binding protein A12; MMP9, matrix metalloproteinase 9.