Table 3.
Defense response genes altered in peripheral blood mononuclear cells 6 h after intravenous endotoxin challenge
| Probe Set and Gene Name | Fold Change 0–6 h (95% LCL, UCL) |
|---|---|
| Pathogen recognition and immune response | |
| 40331_at macrophage receptor with collagenous Structure |
13.6 (9.3, 20.0) |
| 37220_at Fc fragment of IgG, high affinity la, receptor for (CD64) |
9.9 (7.2, 13.6) |
| 31438_s_at CD163 antigen | 7.5 (6.1, 9.2) |
| 32068_at complement component 3a receptor 1 | 4.8 (3.5, 6.6) |
| 37054_at bactericidal/permeability-increasing protein | 4.0 (2.5, 6.4) |
| 37095_r_at formyl peptide receptor-like 1 | 3.9 (2.8, 5.4) |
| 36753_at leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 |
3.1 (2.4, 4.1) |
| 37148_at leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 |
3.1 (2.3, 4.3) |
| 40310_at toll-like receptor 2 | 2.7 (2.2, 3.4) |
| 35094_f_at leukocyte immunoglobulin-like receptor, Subfamily A (without TM domain), member 3 |
2.7 (2.3, 3.2) |
| 37689_s_at Fc fragment of IgG, low affinity IIa, receptor for (CD32) |
2.6 (1.7, 3.8) |
| 36889_at Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide |
2.5 (2.1, 3.1) |
| 36661_s_at CD14 antigen | 2.5 (1.9, 3.3) |
| 35036_at complement component 1, q subcomponent, receptor 1 |
2.5 (1.6, 3.8) |
| 38533_s_at integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170)) |
2.3 (1.7, 3.2) |
| 39221_at leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 |
2.2 (1.6, 3.1) |
| 39997_at properdin P factor, complement | 2.2 (1.6, 3.2) |
| 34665_g_at Fc fragment of IgG, low-affinity IIb, receptor for (CD32) |
2.1 (1.5, 2.8) |
| 36243_at toll-like receptor 1 | 2.0 (1.5, 2.6) |
| 37799_at asialoglycoprotein receptor 2 | 2.0 (1.4, 2.7) |
| 34033_s_at leukocyte immunoglobulin-like receptor, Subfamily A (with TM domain), member 2 |
1.9 (1.4, 2.7) |
| 37470_at leukocyte-associated Ig-like receptor 1 | 1.9 (1.6, 2.3) |
| Chemokine/cytokine receptors | |
| 37603_at interleukin 1 receptor antagonist | 4.7 (3.1, 7.1) |
| 39994_at chemokine (C-C motif) receptor 1 | 3.0 (2.1, 4.2) |
| 41140_at interferon gamma receptor 2 (interferon gamma transducer 1) |
3.0 (2.3, 3.9) |
| 36229_at interleukin 17 receptor | 2.5 (1.9, 3.4) |
| 41677_at interleukin 15 receptor, alpha | 2.5 (1.8, 3.5) |
| 38969_at interleukin 27 | 2.4 (1.9, 3.0) |
| 39938_g_at chemokine (C-C motif) receptor 2 | 2.2 (1.7, 2.9) |
| 33227_at interleukin 10 receptor, beta | 2.1 (1.6, 2.7) |
| 39424_at tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) |
0.7 (0.5, 0.9) |
| 35659_at interleukin 10 receptor, alpha | 0.6 (0.4, 0.8) |
| 1097_s_at chemokine (C-C motif) receptor 7 | 0.5 (0.3, 0.8) |
| 1897_at transforming growth factor, beta receptor III (betaglycan, 300kD) |
0.4 (0.3, 0.6) |
| 1404_r_at small inducible cytokine A5 (RANTES) | 0.4 (0.2, 0.7) |
| 34077_at G protein-coupled receptor 9 | 0.4 (0.3, 0.5) |
| 40729_s_at lymphotoxin beta (TNF superfamily, member 3) |
0.4 (0.3, 0.6) |
| 38578_at tumor necrosis factor receptor superfamily, member 7 |
0.3 (0.2, 0.5) |
| 1370_at interleukin 7 receptor | 0.3 (0.2, 0.5) |
| 31496_g_at small inducible cytokine subfamily C, member 1 (lymphotactin) |
0.3 (0.2, 0.5) |
| 34023_at Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide |
0.1 (0.1, 0.2) |
| Growth factors | |
| 37494_at colony-stimulating factor 2 receptor, beta, low affinity (granulocyte-macrophage) |
4.1 (2.0, 8.4) |
| 32675_at bone marrow stromal cell antigen 1 | 3.0 (2.4, 3.7) |
| 36879_at endothelial cell growth factor 1 (platelet- derived) |
2.9 (2.1, 4.1) |
| 39061_at bone marrow stromal cell antigen 2 | 2.7 (2.1, 3.5) |
| 33665_s_at colony-stimulating factor 2 receptor, alpha, low affinity (granulocyte-macrophage) |
2.2 (1.5, 3.3) |
| 1953_at vascular endothelial growth factor | 1.7 (1.3, 2.2) |
| Microbicidal oxidase and oxidative stress | |
| 34666_at superoxide dismutase 2, mitochondrial | 9.0 (5.4, 15.0) |
| 38893_at neutrophil cytosolic factor 4 (40kDa) | 3.1 (2.3, 4.1) |
| 37975_at cytochrome b-245, beta polypeptide (chronic granulomatous disease) |
2.4 (1.9, 3.2) |
| 824_at glutathione-S-transferase like; glutathione transferase omega |
1.9 (1.3, 2.8) |
| 35807_at cytochrome b-245, alpha polypeptide | 1.8 (1.6, 2.1) |
| 39729_at peroxiredoxin 2 | 0.6 (0.5, 0.7) |
| Protease and protease inhibitors | |
| 31859_at matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase) |
44.1 (18.6, 104.6) |
| 36984_f_at haptoglobin-related protein | 5.7 (2.8, 11.8) |
| 34761_r_at a disintegrin and metalloproteinase domain 9 (meltrin gamma) |
4.5 (2.9, 7.0) |
| 1693_s_at tissue inhibitor of metalloproteinase 1 | 4.4 (3.5, 5.6) |
| 31891_at chitinase 3-like 2 | 3.8 (2.5, 5.5) |
| 33305_at serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1 |
3.0 (2.3, 4.0) |
| 37021_at cathepsin H | 2.6 (1.9, 3.4) |
| 34876_at carboxypeptidase D | 2.5 (2.1, 3.1) |
| 40712_at a disintegrin and metalloproteinase domain 8 | 2.2 (1.6, 3.1) |
| 36781_at serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase), member 1 |
1.9 (1.5, 2.5) |
| 39581_at cystatin A (stefin A) | 1.9 (1.4, 2.7) |
| Cytotoxin | |
| 36766_at ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin) |
5.2 (3.5, 7.8) |
| 33979_at ribonuclease, RNase A family, 3 (eosinophil cationic protein) |
3.1 (1.9, 5.0) |
| Arachidonate metabolism | |
| 40082_at fatty-acid-coenzyme A ligase, long-chain 2 | 11.5 (7.5, 17.8) |
| 32775_r_at phospholipid scramblase 1 | 5.2 (3.3, 8.0) |
| 37099_at arachidonate 5-lipoxygenase-activating protein | 4.5 (3.4, 6.0) |
| 39799_at fatty acid-binding protein 5 (psoriasis associated) |
2.2 (1.4, 3.5) |
| 39624_at leukotriene b4 receptor (chemokine receptor- like 1) |
2.0 (1.7, 2.4) |
| 1671_s_at mitogen-activated protein kinase 14 | 2.0 (1.4, 2.9) |
| 38099_r_at fatty-acid-coenzyme A ligase, long-chain 4 | 1.8 (1.3, 2.4) |
| 37692_at diazepam-binding inhibitor (GABA receptor modulator, acyl-coenzyme A-binding protein) |
1.8 (1.3, 2.5) |
| Metal-binding proteins | |
| 40456_at upregulated by BCG-CWS | 20.6 (11.8, 36.2) |
| 38598_at solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 |
3.6 (1.6, 8.4) |
| 36130_f_at Homo sapiens, similar to RNA helicase- related protein, clone MGC:9246 IMAGE:3892441 |
2.2 (1.7, 2.9) |
| 33943_at ferritin, heavy polypeptide 1 Coagulation |
1.9 (1.3, 2.7) |
| 35245_at coagulation factor V (proaccelerin, labile factor) |
6.2 (3.7, 10.5) |
| 33803_at thrombomodulin | 2.2 (1.5, 3.2) |
| S100 calgranulins | |
| 34319_at S100 calcium-binding protein P | 15.3 (8.6, 27.0) |
| 38879_at S100 calcium-binding protein A12 (calgranulin C) |
8.4 (5.9, 12.2) |
| 41471_at S100 calcium-binding protein A9 (calgranulin B) |
2.4 (1.9, 3.0) |
| 38138_at S100 calcium-binding protein A11 (calgizzarin) |
2.2 (1.8, 2.6) |
| 41096_at S100 calcium-binding protein A8 (calgranulin A) |
2.0 (1.6, 2.4) |
| T- and B cell-associated genes | |
| 38006_at CD48 antigen (B-cell membrane protein) | 0.7 (0.6, 0.9) |
| 36798_g_at sialophorin (gpL115, leukosialin, CD43) | 0.6 (0.5, 0.7) |
| 40667_at CD6 antigen | 0.5 (0.4, 0.7) |
| 40688_at linker for activation of T cells | 0.5 (0.4, 0.6) |
| 771_s_at CD7 antigen (p41) | 0.5 (0.3, 0.6) |
| 40738_at CD2 antigen (p50), sheep red blood cell receptor |
0.4 (0.3, 0.5) |
| 36277_at CD3E antigen, epsilon polypeptide (TiT3 complex) |
0.4 (0.3, 0.5) |
| 931_at Epstein-Barr virus-induced gene 2 (lymphocyte- specific G protein-coupled receptor) |
0.4 (0.2, 0.6) |
| 1105_s_at T cell receptor beta locus | 0.4 (0.2, 0.5) |
| 32070_at protein tyrosine phosphatase, receptor type, C-associated protein |
0.3 (0.2, 0.5) |
| 41654_at adenosine deaminase | 0.3 (0.2, 0.5) |
| 38147_at SH2 domain protein 1A, Duncan’s disease (lymphoproliferative syndrome) |
0.3 (0.2, 0.5) |
| 41468_at T cell receptor gamma locus | 0.3 (0.2, 0.4) |
| 1498_at zeta-chain (TCR)-associated protein kinase (70 kDa) |
0.3 (0.2, 0.4) |
| 38319_at CD3D antigen, delta polypeptide (TiT3 complex) |
0.3 (0.2, 0.4) |
| 35517_at CD4 antigen (p55) | 0.3 (0.2, 0.5) |
| 32794_g_at T cell receptor beta locus | 0.3 (0.1, 0.5) |
| 432_s_at T cell receptor alpha locus | 0.3 (0.1, 0.5) |
| 1106_s_at T cell receptor alpha locus | 0.3 (0.2, 0.4) |
| 37078_at CD3Z antigen, zeta polypeptide (TiT3 complex) |
0.3 (0.2, 0.4) |
| 38917_at T cell receptor delta locus | 0.3 (0.2, 0.4) |
| 39226_at CD3G antigen, gamma polypeptide (TiT3 complex) |
0.2 (0.1, 0.3) |
| 1365_at interleukin 2 receptor, beta | 0.1 (0.1, 0.3) |
| Cytotoxic and NK cells | |
| 39239_at CD8 antigen, beta polypeptide 1 (p37) | 0.5 (0.4, 0.7) |
| 39119_s_at natural killer cell transcript 4 | 0.4 (0.2, 0.6) |
| 36887_f_at killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 |
0.3 (0.2, 0.8) |
| 36280_at granzyme K (serine protease, granzyme 3; tryptase II) |
0.3 (0.2, 0.5) |
| 36777_at DNA segment on chromosome 12 (unique) 2489 expressed sequence |
0.3 (0.2, 0.5) |
| 36886_f_at killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3 |
0.3 (0.2, 0.6) |
| 32297_s_at killer cell lectin-like receptor subfamily C, member 2 |
0.3 (0.2, 0.6) |
| 32904_at perforin 1 (pore-forming protein) | 0.3 (0.2, 0.5) |
| 40699_at CD8 antigen, alpha polypeptide (p32) | 0.3 (0.2, 0.5) |
| 40718_at cathepsin W (lymphopain) | 0.3 (0.2, 0.3) |
| 32370_at similar to granzyme B (granzyme 2, cytotoxic T lymphocyte-associated serine esterase 1) |
0.2 (0.1, 0.4) |
| 32287_s_at killer cell lectin-like receptor subfamily C, member 3 |
0.2 (0.1, 0.4) |
| 35449_at killer cell lectin-like receptor subfamily B, member 1 |
0.2 (0.1, 0.3) |
| 40757_at granzyme A (granzyme 1, cytotoxic T lymphocyte-associated serine esterase 3) |
0.2 (0.1, 0.3) |
| 37145_at granulysin | 0.1, (0.1, 0.3) |
| 32264_at granzyme M (lymphocyte met-ase 1) | 0.1 (0.0, 0.2) |
| MHC and APC | |
| 32035_at major histocompatibility complex, class II, DR beta 4 |
0.7 (0.1, 1.2) |
| 36270_at CD86 antigen (CD28 antigen ligand 2, B7–2 antigen) |
0.6 (0.4, 0.7) |
| 36878_f_at major histocompatibility complex, class II, DQ beta 1 |
0.4 (0.3, 0.7) |
| 38833_at major histocompatibility complex, class II, DP alpha 1 |
0.4 (0.3, 0.5) |
| 38096_f_at major histocompatibility complex, class II, DP beta 1 |
0.4 (0.3, 0.5) |
| 41609_at major histocompatibility complex, class II, DM beta |
0.3 (0.3, 0.4) |
| 36155_at KIAA0275 gene product | 0.3 (0.2, 0.5) |
| Apoptosis | |
| 33727_r_at tumor necrosis factor receptor superfamily, member 6b, decoy |
6.0 (2.9, 12.7) |
| 2002_s_at BCL2-related protein A1 | 4.8 (3.0, 7.6) |
| 1497_at lymphotoxin beta receptor (TNFR superfamily, member 3) |
3.7 (2.0, 6.9) |
| 33412_at lectin, galactoside binding, soluble, 1 (galectin 1) |
2.3 (1.8, 2.9) |
| 1563_s_at tumor necrosis factor receptor superfamily, member 1A |
1.9 (1.6, 2.3) |
| 32725_at BH3-interacting domain death agonist | 1.9 (1.4, 2.5) |
| 2066_at BCL2-associated X protein | 1.8 (1.5, 2.2) |
| 31536_at reticulon 4 | 1.8 (1.5, 2.1) |
| 37643_at tumor necrosis factor receptor superfamily, member 6 |
1.7 (1.3, 2.3) |
| 41189_at tumor necrosis factor receptor superfamily, member 12 |
0.6 (0.5, 0.8) |
| 31491_s_at caspase 8, apoptosis-related cysteine protease |
0.6 (0.4, 0.8) |
| 849_g_at TNF receptor-associated factor 1 | 0.6 (0.4, 0.7) |
| 35238_at TNF receptor-associated factor 5 | 0.5 (0.4, 0.6) |
| 37127_at death effector filament-forming Ced-4-like apoptosis protein |
0.5 (0.3, 0.8) |
| 1847_s_at B cell CLL/lymphoma 2 | 0.5 (0.3, 0.7) |
| 32967_at regulator of Fas-induced apoptosis | 0.4 (0.3, 0.5) |
LCL, lower confidence limit; UCL, upper confidence limit; NK cells, natural killer cells; MHC, major histocompatibility complex; APC, antigen presenting cell.