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. 2017 Aug 17;12(8):e0183298. doi: 10.1371/journal.pone.0183298

Fig 4. Interaction between KIF4 and condensin I is independent on Aurora B kinase activity.

Fig 4

(A) HEK293T cells expressing GFP-hCAPH-wt or GFP-hCAPH-S70A were immunostained and z-stack images were obtained. After deconvolution process, projection images were prepared. Scale bar, 10 μm. (B) Western blot analysis of whole cell extract, chromatin fraction and cytoplasmic fraction prepared from mitotic HEK293T cells expressing GFP-hCAPH-wt or GFP-hCAPH-S70A. Tubulin and histone H3 were used as loading controls. (C) The band intensity of (B) was quantified using ImageJ software and normalized against protein amount in whole cell extract of GFP-hCAP-H-wt expressing cells. n = 3. (D) Physical interaction between GFP-hCAP-H-wt or GFP-hCAP-H-S70A and KIF4 in HEK293T cells. GFP-tagged hCAP-H were immunoprecipitated from chromatin-bound protein fractions of the cells as indicated. Co-immunoprecipitation of GFP-hCAP-H-wt or GFP-hCAP-H-S70A with KIF4 was analyzed by western blot. n = 3.