Fig 2. Ovarian function influences insulin-dependent attractiveness.

(A) Removal of vitellogenic ovaries reverses attractiveness of flies with globally manipulated insulin signaling. Introducing a single copy of ovo^D1 into transgenic flies blocks egg development at early previtellogenic stages, and in flies with global (gsTub5-Gal4) insulin signaling manipulations, it reverses the effects on attractiveness. (B) Manipulation of insulin signaling in late follicle cells (C204-Gal4,TubP-Gal80^ts) is sufficient to affect attractiveness. Opposing insulin signaling manipulations in the germline (Nos-Gal4,TubP-Gal80^ts, first panel) or early follicle cells (C587-Gal4, TubP-Gal80^ts, middle panel) did not produce opposing effects on attractiveness, while manipulations in late follicle cells recapitulated effects of global insulin signaling manipulations (right panel).