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NSG mice (n = 16) were i.p. injected with 40 mg/kg IM. Blood samples were obtained pre-dose and at 5, 15, 30, 60, 120, 180, 240 and 300 minutes after injection using a sparse sampling design (n = 2 samples per mouse, n = 4 samples per time point). Separated plasma was further extracted and processed for analysis, and IM concentrations were determined using HPLC analysis.
