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. 2017 Aug 17;12(8):e0183094. doi: 10.1371/journal.pone.0183094

Fig 5. Brightfield images of ISHH labelling of apela and aplnr transcripts (black grains) in adult rat and mouse kidney.

Fig 5

Apela is largely confined to tubular structures (outline of some arrowed in (A)) whereas aplnr is mainly expressed in intertubular cells (arrowed in (B)) in the rat inner medulla (IM) and outer medulla, respectively. Approx. 35% of cortical glomeruli also expressed aplnr, usually in a uniform distribution across the structure (perimeter of glomerulus arrowed)—an aplnr-positive rat glomerulus is shown in (C)). There is a clear demarcation in overall labelling in tubular structures in the transition from inner medulla (IM) to inner zone of the outer medulla (ISOM) (D) and from the outer medulla (tubular structures arrowed in the outer stripe of the outer medulla (OSOM)) to the cortex (CX) (E) in mouse kidneys. In D and E the insets are approximately 4-fold magnifications of the wide-field images, showing apela labelling in tubules (one structure arrowed) in the ISOM (D) and isolated cells (from boxed area) in the OSOM (E). Scale bar = 50μm in A-C, and 100μm in D and E.