Table 2. Epitopes selected to be used as synthetic peptides in ELISA test.
|
Peptide Number |
ID (Initial and final coordinates) |
Life stagesa |
Sequence of peptide |
Degree of purity (%) |
Prediction of IDRs (Initial and final coordinates) |
Disorder predictors |
|---|---|---|---|---|---|---|
| 1 | Smp_136560 (1564–1578) | 1 | ITEGNNSREGNSEKV | 60.1 | 1501–1658 | REM465, GlobPipe e IUPred |
| 2 | Smp_141860 (1694–1709) | 1 | NHSMDKDDDDFSDIDK | 95,31 | 1693–1793 | REM465, GlobPipe, IUPred e VSL2B |
| 3 | Smp_093840(219–233) | 2 | TTTNKDDTQINSAPS | 96,69 | 182–239 | REM465, GlobPipe, IUPred e VSL2B |
| 4 | Smp_126160(438–452) | 2 | LVTPESKYYSSLPGN | 95,97 | - | - |
| 5 | Smp_150390.1(216–230) | 2 | SLPSNAHNNDNNSSD | 95,55 | - | - |
| 6 | Smp_167240(213–228) | 2 | QCDLDTQWNPAGTEYS | 97,74 | - | - |
| 7 | Smp_180240(339–353) | 2 | RDWPTTLTGAGGSTT | 97,67 | - | - |
a Life stages of parasite in definitive host
1 –Schistosomulum, lung chistosomulum, adult worm and egg
2—Schistosomulum, lung schistosomulum and adult worm.