Fig 4. The effect of Met on cell death and AMPK signaling pathway in vitro.

Calcein AM/PI double staining was performed in H9c2 cells to determine cell death (A). Quantification of survived cells rate was calculated (B). The protein levels of AMPK/ACC were determined in H9c2 cells (C). *p<0.05 vs. Con group, #p<0.05 vs. IR group, & p<0.05 vs. IR+Met group, $ p<0.05 vs. IR+AICAR group.