Fig. 2.
Effect of 5-HT7R activation by LP-211 on dendritic Ih and synaptic activity. A. Neurolucida reconstruction of a L5 pyramidal cell in the ACC showing the position of the dendritic recording pipette. All recordings in this figure were performed on slices from CCI animals. B. Time course of the dendritic resting membrane potential (Vm) during bath application of the 5-HT7 agonist LP-211 (27 nM) from the cell shown in B. Summary bar graph shows average values. C. Example traces of the voltage response induced by a hyperpolarizing current step during baseline (red) and in the presence of LP-211 (blue) in a CCI animal. The average sag ratio showed a significant increase after bath application of LP-211 (n = 5). D. Time course of the EPSP amplitude during bath application of LP-211. Synaptic potentials were evoked by extracellular stimulation of the inner layer 1. Example traces show averaged EPSPs before and after the application of LP-211. E. EPSP amplitude and coefficient of variation (CV2) were not affected by LP-211 whereas the EPSP time integral was reduced (n = 10). F. Paired EPSPs evoked at 50 Hz before and in presence of LP-211. Bar graph shows that the paired-pulse ratio was reduced by LP-211, indicating a reduction in temporal summation (n = 10). Error bars are SEM. *p < 0.05, **p < 0.01.