Fig 1. Decreased intracellular inositol levels in mck1Δ cells.

(A) Cells were grown in SC medium to the early stationary phase. Intracellular inositol levels were determined as described in “Experimental procedures” (3 independent experiments with 3 replicates each). Values shown are mean ± SEM. (B) Inositol restored the growth of mck1Δ cells at elevated temperature. WT and mck1Δ cells were diluted and plated on SC plates in the presence or absence of 75 M inositol and incubated at indicated temperatures for 2 days.