Table 1. Different esterification conditions for BMIPP or BDP-C16.

Yields tested by injection of the crude reaction mixture into HPLC and comparison of the esterified vs. the non-esterified FA signal plus byproducts.

Fatty acid	Coupling agent	Solvent	Rec. volume [μL]	Activator	Temperature [°C]	Reaction time [min]	Yield [%]	n
BMIPP	1,3-Diolein	Toluene	50	SOCl2	70	30	72	4
BMIPP	1,3-Diolein	Acetonitrile	50	SOCl2	70	30	0.6	1
BMIPP	1,3-Diolein	Diethylether	50	SOCl2	70	30	67	1
BMIPP	1,3-Diolein	Tetrachlormethane	50	SOCl2	70	30	8	1
BMIPP	1,3-Diolein	Benzene	50	SOCl2	70	30	38	1
BMIPP	1,3-Diolein	Toluene	50	Oxalyl Chloride	70	30	43	1
BMIPP	1,3-Diolein	Toluene	50	Oxalyl Chloride	70	60	44	1
BMIPP	1,3-Diolein	Toluene	50	SOCl2	70	90	54	2
BMIPP	1,3-Diolein	Toluene	50	SOCl2	70	120	67	2
BMIPP	1,3-Diolein	Toluene	50	SOCl2	70	150	86	2
BMIPP	EtOH	MeCN	100	SOCl2	0	30	1	1
BMIPP	EtOH	MeCN	100	SOCl2	22	30	64	1
BMIPP	EtOH	MeCN	100	SOCl2	70	30	88	1
Bodipy C16	1,3-Diolein	Toluene	200	SOCl2	70	30	59	1
Bodipy C16	1,3-Diolein	Toluene	50	SOCl2	70	30	95	8