Fig 1. Effect of diflunisal on the NLC in HEKs expressing prestin.
(A) Representative non-linear capacitance curves recorded on the same HEK cell expressing prestin, with (○) or without (●) DFL. (B). Representative effect of DFL on the capacitance vs. voltage curve in the absence of prestin in HEKs. The membrane capacitance CM of non-transfected HEKs was monitored at voltages from– 160 mV to +160 mV in the absence and presence of DFL. (C) Effect of DFL on the membrane capacitance in the presence (Clin) and absence (CM) of prestin. Both Clin and CM were obtained from fits to the capacitance vs. voltage curves, using Eq 1 or a linear curve respectively. N = 4 for each condition. The error bars show the standard deviation. (D) Fold increase for each parameter obtained from fitting the NLCs to Eq 1; n≥3 cells for each condition. The error bar shows the standard deviation. The t-test is conducted between the values obtained upon perfusion of ECB with DFL versus ECB alone: * p<0.01; ** p<0.001.